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. 2016 Apr 21;35(12):1276–1297. doi: 10.15252/embj.201593350

Figure 4. Reduced expression of C9ORF72 promotes aggregation of TDP‐43.

Figure 4

  1. Immunoblot analysis of endogenous Tdp‐43, C9orf72, and control Gapdh of E18 mouse cortical neurons transduced with lentivirus expressing either control shRNA or shRNA targeting C9orf72 mRNA.
  2. Left panel, representative images of immunofluorescence labeling of endogenous phosphorylated Ser409/410‐Tdp‐43 on primary cultures of E18 mouse cortical neurons transduced with lentiviral particles expressing either control shRNA or shRNA targeting C9orf72. Right panel, quantification of cytoplasmic Tdp‐43 aggregates.
  3. Left panel, representative images of immunofluorescence labeling of D169G mutant GFP‐tagged TDP‐43 on primary cultures of E18 mouse cortical neurons transfected with either HA‐tagged control or HA‐C9ORF72 plasmid. Right panel, quantification of cells with cytoplasmic aggregates of TDP‐43.
Data information: Scale bars, 10 μm. Nuclei were counterstained with DAPI. Error bars indicate SEM. Student's t‐test, ***P < 0.001, n = 3. Source data are available online for this figure.