Figure 1.

3D architecture confers decreased sensitivity to platinum in colorectal cancer. (A-B) HCT116 and LoVo cultured on plastic grew as flat monolayers (left) and formed multicellular spheroids (middle) using the liquid overlay technique. Multicellular spheroids sections were stained with H&E (right). (C) WST assay indicated that 3D cultures were less sensitive to platinum compared with 2D cultures. *:p<0.01 (D) After 3D or 2D cultures treated with or without CDDP, cells were detached and cultured as monolayer cells for 7 days and were subsequently stained with crystal violet. The clonogenicity of the 3D cultures treated with CDDP was much higher than that of the respective 2D cultures.