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. 2016 Apr 29;7(8):900–909. doi: 10.7150/jca.14506

Figure 4.

Figure 4

JNK1/2 do not play a dominant role in the 3D architecture-mediated p53-dependent decrease in chemosensitivity. (A) MAPKs (JNK1/2, p38 and ERK1/2) were activated by CDDP in a time-dependent manner. Knockdown of p53 decreased CDDP's activation of JNK1/2, but not p38 or ERK1/2 (western blot). (B) Cells were treated with vehicle or SP600125 1 hour prior to CDDP treatment. CDDP's activation of JNK1/2 was remarkably inhibited by SP600125. Inhibition of JNK1/2 activation did not detectably change CDDP's induction of p53 (western blot). (C) Cells were treated with vehicle or SP600125 1 hour prior to CDDP treatment. Cell viability was assayed using WST. Pretreatment with 20 μM SP600125 significantly increased chemosensitivity. *:p<0.05.