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. 2016 May 12;7(8):935–946. doi: 10.7150/jca.13628

Figure 1.

Figure 1

Correlation of HBV with expression of AFP, Ras and CXCR4 in HCC tissues. Clinical liver tissues sample were collected after surgical hepatectomy. A, Expression of AFP, Ras and CXCR4 in the tissues were detected by immunohistochemistry assay. B, Concentration of serum AFP was detected by ELISA. 1, Normal; 2, Hepatitis patients(HBV+); 3, Non metastasis HCC patients(HBV+); 4, Lymph nodes metastasis HCC patients(HBV+). C, Expression of AFP, Ras and CXCR4 in the tissues were detected by Western blotting, right column images represented the proteins densitometry value ratio compared with internal control β-actin. 1, Normal liver tissues; 2, Hepatitis tissues(HBV+); 3, Non metastasis HCC tissues(HBV+); 4, Lymph nodes metastasis HCC tissues(HBV+). The images representation of at last three reduplicate experiments. HE stained: Haematoxylin and eosin stained; IH: Immunohistochmeistry stained.