FIGURE 3.
ELMO1/Dock1 complex interacts with Gβ upon GPCR activation. A. ELMO1/Dock1 complex interacts with Gβ subunit upon chemoattractant stimulation determined by immunoprecipitation using anti-GFP antibody conjugated magnetic beads as bait. Western-blot detection of Dock1, RhoG, and Gβ is also shown. B. Quantitative measurement of the interaction of ELMO1 and its interacting partners in panel A. Mean ± SD from two independent experiment is shown. C. Montage shows a decreased membrane translocation of YFP-ELMO1 (Green) in PT treated HeLa cells in response to SDF1α stimulation. Fluorescent dye Alexa 633 (Red) containing 100 ng/ml SDF1α was uniformly applied to HeLa cells.