Figure 4.

Deguelin down-regulated the expression of galectin-1 in lung SCC cells. (A, B) NCI-H520 and SK-MES-1 cells were treated with various concentrations of deguelin for 24 hours. Total cellular RNA was extracted and analysed using real-time PCR. The data from three independent experiments are presented as mean±SD. **P<0.01 vs Control. (C, D) NCI-H520 and SK-MES-1 cells were incubated with different concentrations of deguelin for 24 hours, cell lysates were harvested and the indicated proteins were determined by western blotting.