Figure 6.

Deguelin induced apoptosis of lung SCC cells in a galectin-1 dependent manner. (A, B) Both galectin-1 overexpression and knockdown cells were incubated with 25μM deguelin for 72 hours and then measured cell viability using WST-8 assays. (C, D) Galectin-1 overexpression or knockdown cells were seeded in six-well plates. After 24 hours of incubation, the cells were treated with 10μM deguelin for 14 days. Then the colony formation efficiencies were summarized. (E) Galectin-1 overexpression or knockdown cells were incubated with 25μM deguelin for 24 hours. Flow cytometry was used for cell apoptosis analysis. (F) Galectin-1 overexpression or knockdown cells were incubated with 25μM deguelin for 24 hours. Cell lysates were harvested and the indicated proteins were determined by western blotting. All data are from three independent experiments and presented as mean±SD. *P<0.05, **P<0.01.