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. 2016 Jun 21;22(23):5364–5373. doi: 10.3748/wjg.v22.i23.5364

Figure 2.

Figure 2

Tg737 is a target gene of miR-548a-5p. A: MiR-548a-5p and its deductive binding domain in the 3'UTR of Tg737; B: Relative expression of miR-548a-5p; C: qRT-PCR and Western blot analysis demonstrated that miR-548a-5p overexpression significantly decreased Tg737 mRNA expression and miR-548a-5p knockdown significantly increased Tg737 mRNA expression in HepG2 and MHCC97H cells; D: Luciferase reporter assay of the connection between the 3'UTR of Tg737 and miR-548a-5p in HepG2 and MHCC97H cells; E: Western blot analysis demonstrated higher miR-548a-5p and lower Tg737 expression in human HCC specimens compared to normal liver tissue; F: qRT-PCR demonstrated higher miR-548a-5p and lower Tg737 expression in human HCC specimens compared to normal liver tissue; G: Spearman’s correlation assay showed that miR-548a-5p was negatively correlated with Tg737 mRNA expression in HCC specimens. aP < 0.05, bP < 0.01 vs control.