Extended data figure 3.

(A-G) Dietary emulsifiers alter gut microbiota composition in WT mice relative to littermate controls. All the female (n=33, A-B) and male (n=33, C-D) mice from 10 different litters were placed into cages in a manner such that each litter was split equally amongst the 3 experimental groups that were to receive water, CMC or P80 (3 cages per sex per condition). Mice were exposed to drinking water containing CMC or P80 (1.0%) for 8 weeks. (A, C) Day 0 and (B, D) day 63 PCoA of the unweighted UniFrac distance matrix of fecal microbiota in WT mice. Treatment of each mouse is indicated by point color (blue = water; orange = CMC; purple = P80). Mother of each mouse is indicated by point color. Cage of each mouse is indicated by point color. (E-F) Mice were clustered using UPGMA (Unweighted Pair Group Method with Arithmetic mean) method. Treatment of each mouse is indicated by line color (blue = water; orange = CMC; purple = P80). (G) Schematic representation of the above experimental design. (H-N) Dietary emulsifiers alter gut microbiota composition in TLR5^−/− mice relative to littermate controls. All the female (n=25, H-I) and male (n=23, J-K) mice from 8 different litters were placed into cages in a manner such that each litter was split equally amongst the 3 experimental groups that were to receive water, CMC or P80 (2 cages per sex per condition). Mice were exposed to drinking water containing CMC or P80 (1.0%) for 8 weeks. (H, J) Day 0 and (I, K) day 63 PCoA of the unweighted UniFrac distance matrix of fecal microbiota in TLR5^−/− mice. Treatment of each mouse is indicated by point color (blue = water; orange = CMC; purple = P80). Mother of each mouse is indicated by point color. Cage of each mouse is indicated by point color. (L-M) Mice were clustered using UPGMA (Unweighted Pair Group Method with Arithmetic mean) method. Treatment of each mouse is indicated by line color (blue = water; orange = CMC; purple = P80). (N) Schematic representation of the above experimental design.