Figure 2.

Multi-functionality and cytotoxicity of in vitro primed T cells reacting to RMLPHAPGV. (A) T cells show significant expression of TNFα, MIP-1β, IL-2 and CD107a after 6 h incubation with RMLPHAPGV. Numbers in the plot reflect percentage of reactive T cells for the respective marker. (B) Reactive T cells show different degrees of functionality. Only T cells expressing at least one activation marker (60.1% of all RMLPHAPGV-specific T cells) are displayed. 39.9% of RMLPHAPGV-tetramer-specific T cells did not react upon peptide stimulation. (C) Different combinations of activation markers are represented within the reactive T-cell population. (D) Percentage of specific lysis by antigen-specific T cells was determined by ⁵¹chromium release assay after 24 h coincubation with different RMLPHAPGV peptide-loaded or peptide-unloaded OvCa cell lines or HLA-negative K562 cells. Experiments were performed in triplicates with a 30:1 E:T ratio.