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. Author manuscript; available in PMC: 2016 Jun 16.
Published in final edited form as: Mamm Genome. 2008 Sep 3;19(9):623–633. doi: 10.1007/s00335-008-9138-5

Figure 3. The mutation causes a reduction of wild-type messages in mutant animals.

Figure 3

Panel A shows RT-PCR of RNA from C57BL/6J (wt), heterozygous (+/−) and homozygous mutants (−/−). The 25bp insertion into the message is seen at albeit reduced levels. Panel B depicts relative expression of Prph2 using a primer that detects only wild-type message in a quantitative RT-PCR assay. Expression is given as number of molecular per 1000 molecules of β-actin, genotype is indicated on the x-axis.