Figure 6.

Gene structure of Tpcn2 mutant mouse lines. Organization of figure as for Figure 5. Symbols for mutant alleles are based on MGI database nomenclature, except for Tpcn2^{Grimm et al.} which is indicated by the publication reference where it was first described. (A) Gene trap line Tpcn2^{Gt(YHD437)Byg} (gene trap vector pGT0Lxf, inserted in intron upstream from exon 1). (B) Targeted homologous recombination line Tpcn2^tm1Lex (targeted deletion of exons 18–20 with knock out cassette from a pKO vector). (C) Cre loxP line Tpcn2^{Grimm et al.} (Cre-mediated excision of floxed exon 7). (D) Cre loxP line Tpcn2^tm1.1Dren (Cre-mediated excision of floxed exon 1 containing the initiation codon). Puro^R (cDNA for puromycin N-acetyl-transferase); all other abbreviations and symbols as in Figure 5.