Figure 2.

TPL reduced the infiltrated AMs and inhibited the production of ROS from AMs-IR. (A) Macrophage in lung tissue was detected by immunohistochemistry (×400) and Flow cytometry (B). (C) Cultured AMs-ctrl and AMs-IR were treated with or without Apocynin (Apo, 300 μM) or TPL (5 ng/ml) (n = 5, in duplicate) for 6 h and 12 h, and ROS production was measured by DCFH-DA fluorescence assay. Values are expressed as mean ± sd, relative to Ctrl. **P< 0.01 vs. ctrl vehicle; ##P < 0.01 vs. IR vehicle. (D) ROS production was measured in irradiated MH-S cells with or without pretreated by Apocynin or TPL for 4 h. * P < 0.05 vs. Ctrl; # P < 0.05 vs. 2 Gy.