Figure 4.

RO4929097 reduces number and activity of OCL. (A,B) Peripheral blood MNC obtained from 5 patients with MM were differentiated toward OCL with or without the continuous presence of 1 µM RO4929097. (A) TRAP staining was performed. Representative micrographs are shown (magnification, x20). (B) Cells were plated on Corning Osteo Assay Surface 24-well plates and differentiated into OCL. Resorption was evaluated 14 d after the start of the cultures. (Left) Representative micrographs are shown (magnification, x2). (Right) Resorption area was then measured and its proportion was calculated. Shown are combined results obtained from 4 different BM samples. (C) Primary CD138⁺ MM cells (n = 16) isolated from BM of patients with MM were kept with or without 1 µM RO4929097 for 24 hr. The expression of indicated genes was determined by qPCR. Shown are fold changes in expression of indicated genes in treated cells as compared to control untreated cells (presented as a horizontal line).