Figure 2.

Cell cycle perturbations induced by ganetespib on HCC cells in vitro. Synchronized cells were exposed to vehicle control or 20 nM ganetespib for 0, 24, or 48 hours and then fixed with ethanol for cell cycle analysis with propidium iodide. Treatment with ganetespib caused a G₂-M arrest in all cell lines as can be seen by the increase in percentage of cells in the G₂-M phase at 24 and 48 hours when compared to vehicle controls for (A) Hep3b, (B) HepG2, and (C) HUH7 cell lines. Percent of cells in G₁, S, G₂ and G₂–M phases is plotted for the control and ganetespib arms. (D) The sub-G1 population of cells as shown by flow cytometry of HCC cells treated with ganetespib (20 nM) or vehicle control for Hep3b, HepG2 and HUH7 cell lines. All experiments were done in triplicate and repeated at least twice. Error bars indicate SD. The * - p < 0.05, ** - p < 0.01 and *** - p < 0.001.