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. 2016 Mar 22;17(4):439–448. doi: 10.1080/15384047.2016.1156255

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© 2016 National Research In Reproductive Health, Indian Council Of Medical Research

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Figure 8. — EGR1 silencing and its effect on hTERT expression in DU145 cells. qRT-PCR (A) and immunoblotting (B) were done to assess the efficiency of EGR1 silencing . Panel C represents mean ratios of the intensities of EGR1 to that of GAPDH bands in the cells transfected with either scrambled oligos (SO) or EGR1 siRNA (silenced). Panel D represents immunofluorescent detection of EGR1 protein in SO (a) and EGR1 (c) siRNA transfected cells. Panel e shows the cells stained only with secondary antibody. Lower panels -b, d and f are the DAPI stained images of the cells shown in panels a, c and e, respectively. Magnification-63X. Panel E shows relative levels of hTERT transcripts in cells transfected with SO and EGR1 siRNA. * p value <0.05, **** p value< 0.0001.