Figure 2.

p38-MAPK activation led to the evasion of apoptosis induced by VX680. (A) HeLa cells were treated with various concentrations of VX680 for 72 h, and apoptosis was then analyzed by flow cytometry. Annexin V + was identified as apoptosis. (B) Results of Annexin V assays for apoptosis detection. The graphs illustrate the means ± the SDs of triplicate results. (C) HeLa cells were treated with various concentrations of VX680 for 48 h. The expressions of related proteins were determined by immunoblotting. (D) HeLa cells were treated with various concentrations of VX680 and cultured for 24, 48, or 72 h. The expressions of p38-related proteins were determined by immunoblotting. GAPDH served as a loading control.