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. 2016 Mar 30;17(5):546–557. doi: 10.1080/15384047.2016.1167292

Figure 4.

Figure 4.

The effect of Mitoxantrone on TRAIL-resistant subpopulation, U87MG-R50. (A) The difference of U87MG cells and U87MG-R50 cells response to different doses (50, 250 and 500 ng/ml) of TRAIL. (B) Scatter plot of U87MG cell viability upon 24 hours of treatment with drug library composed of 1200 compounds and TRAIL together. Each dot represents the combinatorial effect of the individual drug (5 µM) and TRAIL (25 ng/ml) on cell viability compared to untreated control samples. Horizontal dashed lines depict the range of viability within 3 SD of the mean viability (41% and 113%). Red and blue dots indicate the combination effect below the threshold of 3 SD from the mean cell viability. (C) The effect of Mitoxantrone (500 nM) and/or TRAIL (50 ng/ml) on U87MG-R50 cells. (D) Detection of Caspase-8, −9 and −3/7 activity levels of Mitoxantrone and/or TRAIL treated U87MG-R50 cells. * indicates p <0.05 and ns indicates nonsignificant (p>0.05).