Figure 4.

Internalization and trafficking of hertuzumab-MMAE to the lysosome in NCI-N87 cells. N87 cells were incubated with hertuzumab-MMAE on ice or 37°C. Hertuzumab-MMAE was detected using Alexa Fluor 488-labeled goat antihuman IgG and are shown in green. Lysosomes were visualized using a mouse anti-human Lamp-2 antibody followed by Alexa-Fluor®647 labeled goat anti-mouse IgG and are shown in red. DAPI-stained nuclei are in blue. The orange signal in the merged image represents the hertuzumab-MMAE in the lysosome