Figure 3.

Direct binding of c-Jun to the promoter/enhancer regions of FosB, NFATC2, WEE1, PVR, LGALS3 and MAP1LC3B. ChIP assays demonstrate direct binding of c-Jun to the promoter/enhancer regions of the detected c-Jun target genes. DNA samples from the ChIP reactions (Pol II, IgG, and c-Jun) and input DNA were used for PCR with different primer pairs to amplify specific promoter regions (ChIP_FosB, ChIP_NFATC2, ChIP_WEE1, ChIP_PVR, ChIP_LGALS3 and ChIP_MAP1LC3B). All PCR fragments were detected in the input DNA sample. The bars show the means±s.d. of three independent experiments; measurements were performed in triplicate (**P < 0.01; *P < 0.5).