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. 2016 Mar 8;17(5):498–506. doi: 10.1080/15384047.2016.1156266

Figure 1.

Figure 1.

Expression of STD fusion protein and construction of the complex (a) Expression and identification of STD fusion protein. SDS–PAGE stained with Coomassie brilliant blue (left graph). Lane M: standard molecular weight markers of protein; Lane 1: ultrasound supernatant of E. coli Rosetta-gami transformed with pET-44b-STD; Lane 2: purified STD protein corresponding to Lane 1. Western Blotting analysis of purified STD protein with anti-TAT or anti-DRBD antibody (right graph). (b) EMSA analysis of interaction between siRNA or A10 and STD fusion protein. Ten μL siRNA (1/32 μM) and 10 μL A10 (1/4 μM) pre-incubated with serial 2-fold diluted STD protein. Untreated siRNA or A10 was used as mock control and Bovine Serum Albumin (BSA) as negative control. (c) Schematic diagram of assembled A10-STD-(sur-siRNA) complex.