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. 2016 Mar 8;17(5):498–506. doi: 10.1080/15384047.2016.1156266

Figure 4.

Figure 4.

Down regulation of SURVIVIN gene and its effect on apoptosis of LNCaP cells (a) Expression analysis of SURVIVIN gene. Real-time quantitative PCR (left graph) was used to detect the expression of SURVIVIN mRNA. Relative amount of SURVIVIN mRNA was normalized to β-actin mRNA applying 2−△△CT algorithm. Relative expression of untreated LNCaP cells was normalized as 100%. Data represent Mean +/− SEM of at least 3 separate experiments. Comparison was between the complex group with other groups. ***: P < 0.0001; **: P < 0.001; *: P < 0.01. Western blotting (right graph) was used to detect the expression of SURVIVIN protein corresponding to mRNA. (b) Flow cytometry analysis of apoptosis induced by down-regulation of SURVIVIN gene using Annexin V assay. The histogram showed the sur-siRNA transfected group with blank bars, while the parallel group with sur-siRNA and cisplatin was showed with slash bars. The percentage of total apoptosis (early and late) of LNCaP cells was showed above the bars.