The cysteine mutation at N573 allowed efficient biotinylation to the chimeric VLP than the other mutation sites, in other words, Y485, T489, S533 and T586, although those mutations retained similar in biotinylation after VLP disassembly (A). Both the wild-type VLP and the chimeric VLP were recognized by an antibody, HEP40-4B, with similar reactivity, while chimeric VLP was unreactive to antibody, HEP230 (B).
CBS: Coomassie Blue staining; VLP: Virus-like particle.