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. 2016 Jun 16;11(6):e0156486. doi: 10.1371/journal.pone.0156486

Fig 2. Up-regulation of mRNA of different innate inhibitors of viral replication in primary human macrophages treated with ProTα variants.

Fig 2

A) INF-β and IFN-λ1 and (B) TNF-α, IL-6 and RANTES mRNAs induction by ProTα variants. RT-qPCR with specific primers for indicated genes was performed as detailed in Methods on cDNA from primary human macrophages treated for 2 h with 200 ng/ml of ProTα variants or LPS. mRNA transcripts levels were normalized relatively to the expression of the ribosomal protein (RPS11) control mRNA. Each experiment was done at least three times. Data represent mean ± SD. Student’s t test was used to compare means of treated versus control samples. *P < 0.05 was considered to be statistically significant.