Fig 1. Comparison of chloroform/methanol defatted (df) and non-defatted (ndf), buffer D extracted raw and boiled/fried peanut samples.

50μg protein (as determined using the Bradford assay on extracts prior to centrifugation) was loaded per lane on a 14% reducing SDS-PAGE. Lane labeled “spun” was loaded with non-defatted samples that had been centrifuged for one minute at 15,000 rpm in a benchtop eppendorf centrifuge. Arrows indicate differentially detected proteins.