Fig. 2. Env (120–204)–specific IgG is associated with the presence of HLA-DPB1*13 and is protective.

(A) Distribution of IgG stratified by absence or presence of DPB1*13. Red lines represent the mean and SD for the change in the measurement of IgG from absence to presence of DPB1*13. Means were compared using t tests. Individual data points are indicated by black circles. (B) Box plots of vaccinated individuals stratified according to HIV-1 infection status and the absence or presence of DPB1*13. Individual data points are indicated by colored circles within the box plots showing the 25th (bottom edge of the box), 50th (horizontal line in the box), and 75th percentiles (top edge of the box). Analysis of variance tested IgG distributions across subsets with differences further interrogated using Bonferroni-adjusted t tests. (C) Estimated cumulative HIV-1 incidence curves for individuals stratified by IgG and DPB1*13 for the entire vaccinated RV144 cohort. Vaccinated individuals in the case-control study are stratified into subgroups according to their IgG responses (low and high/medium corresponding to the lower one-third and upper two-thirds of responses) at week 26 and the absence or presence of DPB1*13. Individual curves represent the estimated cumulative incidence of HIV-1 infection over time. Curves were estimated using the Kaplan-Meier method with inverse probability weighting accounting for the sampling design. (D) Estimated vaccine efficacy (VE) for IgG and DPB1*13. Individuals were stratified into subgroups according to IgG response (low and high/medium, corresponding to the lower one-third and upper two-thirds of responses) and the absence or presence of DPB1*13 and were compared to the entire placebo-infected group. Points show the estimated VE values, and lines represent 95% confidence intervals (CI) that were estimated using logistic regression models that accounted for the sampling design.