Table 1. Oligonucleotide primers used in this study.
| primers | Sequences(5'-3') | Target | Reference |
|---|---|---|---|
| intI1-R | CAGTGGACATAAGCCTGTTC | class1 integrase | 6 |
| intI1-F | CCCGAGGCATAGACTGTA | ||
| In1-R | AAGCAGACTTGACCTGA | class 1 integron | 8 |
| In1-F | GGCATCCAAGCAGCAAG | ||
| hep74 | CGGGATCCCGGACGGCATGCAC | class 2 integron | 9 |
| hep51 | GATGCCATCGCAAGTACGAG | ||
| int3-F | AGTGGGTGGCGAATGAGTG | class 3 integranse | 10 |
| int3-F | TGTTCTTGTATCGGCAGGTG | ||
| sul1-R | ATTCAGAATGCCGAACACCG | sul1 | 6 |
| sul1-F | TAGCGAGGGCTTTACTAAGC | ||
| sul2-R | GAAGCGCAGCCGCAATTCAT | sul2 | 6 |
| sul2-F | CCTGTTTCGTCCGACACAGA | ||
| dfrA1-R | TTGTGAAACTATCACTAATGGTAG | dfrA1 | 6 |
| dfrA1-F | CTTGTTAACCCTTTTGCCAGA | ||
| dfrA5-R | TCCACACATACCCTGGTCCG | dfrA5 | 6 |
| dfrA5-F | ATCGTCGATATATGGAGCGTA | ||
| dfrA12-R | ATGAACTCGGAATCAGTACGC | dfrA12 | 6 |
| dfrA12-F | TTAGCCGTTTCGACGCGCAT | ||
| dfrA13-R | GAAACTATCACTAATGGCAGC | dfrA13 | 6 |
| dfrA13-F | CTCATCTGCTGGCTATCTCA | ||
| dfrA17-R | TTGAAAATATTATTGATTTCTGCAGTG | dfrA17 | 6 |
| dfrA17-F | GTTAGCCTTTTTTCCAAATCTGGTATG | ||
| dfrA27-R | TAAAGCAATAACTTACAATC | dfrA27 | This study |
| dfrA27-F | AAGAGTCTGATCGCCCATGCCG | ||
| sul2-SP1 | AAAGAACGCCGCAATGTGATCC | 3’flanking nucleotide of sul2 | This study |
| sul2-SP2 | GGATAGAACGCAGCGTCTGGAAAA | ||
| sul2-SP3 | CATCTGCCTTGAGCGCGTCC | ||
| sul2-SP4 | GTGGGAATGAGTTGGAAGAA | 5’flanking nucleotide of sul2 | This study |
| sul2-SP5 | GTTGCCGTTGTGGGTGCTGT | ||
| sul2-SP6 | TGCGCTATCGTGGGGGAATG | ||
| 16s RNA-R | ACGGCAGCACAGAAGAGC | 16s RNA | This study |
| 16s RNA-F | AGTTCGCATCGTTTAGGG |
SP, special primer; F, forward; R, reverse.