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. 2016 Jun 16;11(6):e0157708. doi: 10.1371/journal.pone.0157708

Fig 3. Mutational analysis of HBV cccDNA in HCC patients.

Fig 3

A. Agarose gel analysis of 3D-PCR of cccDNA from CT and CNCT of samples from patients #110, #178, #161and #155. cccDNA was specifically amplified by RCA, then the concatemerized product was analysis by 3D-PCR using a denaturation temperature gradient of 86°C-82°C. B. Mutation matrices of cccDNA from CT and CNCT groups. DNA fragments amplified at 94°C (as reference wild type sequence) or 84.1°C in each group were cloned into T vectors, 13 clones were randomly selected, and the core region was sequenced. C. Dinucleotide editing profile of G to A mutations of plus-polarity strand DNA. Asterisks indicate the 3D-PCR products cloned and sequenced.