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. 2016 Jun 16;11(6):e0157397. doi: 10.1371/journal.pone.0157397

Fig 3. Effects of dasatinib on ER-mediated transcription and ER signalling.

Fig 3

Cell lines were co-transfected with EREIItkLuc and pCH110 and treated with the combinations mentioned (A, B, C, D). Standard concentrations of dasatinib (100nM), E2 (0.01nM), 4-OHT (10nM) and fulvestrant (ICI) (10nM), were used. Normalized luciferase activity from triplicate wells was expressed relative to the vehicle-treated control. Bars represent ± SEM. *p<0.05, **p<0.01, ***p<0.001. Effects were confirmed in three independent experiments. Wt-MCF7, MCF7-LTED, 1%MCF7 and MCF7-TAMR cells were treated as indicated for 24-hours, with the same amounts as stated above. Western blot was used to assess changes in total ER and PgR. Figures below each panel represent semi-quantitave changes in protein expression relative to actin.(E).