Fig 1. Lentivirus-transduced human iPSCs differentiate to multipotent neural precursor cells.

(A) Representative micrographs of human iPSCs transduced with a lentiviral vector containing the Photinus pyralis luciferase gene and puromycin resistance cassette. Photon emission in response to D-luciferin could be detected in transduced puromycin-selected iPSCs (bottom) but not in non-transduced iPSCs (top) in vitro. (B) Representative FACS plots demonstrating CD184+/CD271-/CD44-/CD24+ gating scheme for isolation of NPCs from differentiated iPSCs. (C) Representative dot plots and quantification of NPC marker expression in FACS-sorted EB-NPCs following recovery and propagation (n = 3); data presented as average±SEM.