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. 2016 Jun 16;11(6):e0157620. doi: 10.1371/journal.pone.0157620

Fig 6. Human iPSC-derived NPCs directly induce Treg conversion in vitro.

Fig 6

(A) Representative FACS plots of CD4+FOXP3+ Tregs from EB-NPC-T cell co-cultures. T cells were mixed with EB-NPCs at varying T cell-to-EB-NPC ratios and activated in the presence of anti-CD3/anti-CD28 beads for three days. (B) The frequency and number of FOXP3-expressing CD4+ T cells was significantly increased in the presence of EB-NPCs. (C) The observed increase in Tregs was correlated with a significant decrease in the number of conventional CD4+ T cells at a T cell-to-EB-NPC ratio of 1:4. All data was analyzed using students t-test and is presented as average ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001; n = 4.