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. 2016 Jun 16;11(6):e0157620. doi: 10.1371/journal.pone.0157620

Fig 7. Regulatory T cells are necessary for EB-NPC-induced myelin sparing.

Fig 7

(A) Treatment of EB-NPC-transplanted mice with PC61.5, a rat monoclonal antibody raised against CD25, resulted in a significant reduction in the frequency of circulating CD4+FOXP3+ cells that was sustained to day 21 p.t. (B) Representative spinal cord sections stained with LFB and H&E. Outlined areas highlight demyelination. (C) Quantification of white matter damage revealed PC61.5-treated EB-NPC-transplanted mice did not have reduced demyelination when compared to non-treated EB-NPC-transplanted mice. For (A) and (C), data represents two independent experiments with n = 8 (EB-NPC alone), n = 9 (EB-NPC + IgG control), n = 9 (EB-NPC + PC61.5), and n = 8 (HBSS) animals per group. All data is presented as the average ± SEM and was analyzed using one-way ANOVA followed by Tukey’s multiple comparison test; *p < 0.05, **p < 0.01, ***p < 0.001.