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. 2016 Jun 16;11(6):e0157520. doi: 10.1371/journal.pone.0157520

Fig 5. Expression analysis of vATPase A gene after both RNAi pathway gene dsRNA and vATPase A dsRNA exposures.

Fig 5

Newly emerged WCR adults were injected with 600 ng of dsRNA or equal volume of water and allowed to feed on untreated artificial diet. On day 4 adults were exposed to vATPase A dsRNA as surface treatment on artificial diet. Three days after vATPase A dsRNA exposure, adults were collected and flash frozen in liquid nitrogen and used for vATPase expression evaluation using qRT-PCR. Adults that were only injected with water, GFP dsRNA and laccase2 dsRNA but not exposed to vATPase A dsRNA were included as controls. Standard errors were determined from six independent biological replications (n = 6), each with two technical replications. Different letters within a figure represent significant difference at P value < 0.05.