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. 2015 Apr 27;230(8):1964–1973. doi: 10.1002/jcp.24942

Figure 4.

Figure 4

XBP‐1 is experimentally validated as a direct target of miR‐214 in HUVECs. (A) The sequence of the miR‐214 binding sites within the human XBP‐1 3′UTR and a schematic diagram of the reporter constructs showing the entire XBP‐1 3′UTR sequence. (B) XBP‐1 protein expression in the presence of candidate miRNAs. 100 nM of individual miRNA was introduced into cells. Western blot was performed 48 h after transfection. Blank control culture medium (mock); non‐specific negative control oligonucleotides (negative control); miR‐34c, miR‐214mimics; miR‐34c, miR‐214 inhibitors. (C) XBP‐1 expression in the presence of 100 nM of miR‐214 mimics or miRNA negative control oligonucleotides in H9C2 (2‐1) cells. (D) Real‐time PCR of miR‐214 expression in ISO treated mice after anti‐miR‐214 treatment. Blank control culture medium (mock); non‐specific negative control oligonucleotides (negative control); hsa‐miR‐214 mimics (miR‐214); hsa‐miR‐214 inhibitors (miR‐214 inhibitor).