Fig. 4. The effect of MACF1 knockdown on cellular MTT reduction activity and mitochondrial content. (A) After 24 h, 48 h, and 72 h of cell culture, the MTT assay was conducted, showing no significant difference in the absorbance between the scrambled control and MACF1-KD cells. (B) Detection of the absorbance of the same cell number in the scrambled control or the MACF1-KD group showed increased cellular MTT reduction activity by MACF1 knockdown. MACF1-KD versus scrambled control, *P ＜ 0.05. (C) Cells were stained with MitoTracker green and DAPI, more mitochondrial content (green) was observed in the large binuclear/multinuclear cells. Scale bar = 50 μm.
