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(a) Confirmation of decreased homocysteine abundance in spent media with EtBr-induced mtDNA depletion. n = 3. (b) Hydrogen sulfide levels in cells measured either directly (sulfide) or indirectly by its sulfane products (SSP4). Acute antimycin treatment (1 hr) was used to control for increased H2S levels arising from decreased H2S degradation due to loss of the respiratory chain. n = 6 for both plots.
