TABLE 2.
Summary of differentially expressed genes in the large intestinal tumors of F344 rats chronically exposed to Aloe vera non-decolorized whole leaf extract in drinking water.
| Group | Differentially expressed genes in Aloe vera non-decolorized whole leaf extract–induced tumors |
|---|---|
| MAPK signaling | |
| Ras/Raf1/MEK/ERK | Map2k1, Mapk1 |
| Stress-activated MAPKs | Map2k4, Mapk7, Mapk12, Mapk13, Mapk8ip3 |
| Transcription regulators | Ets1, Tp53, Creb1, Ccne1, Myc, Cdkn2c |
| Cell cycle genes | Ccnd1, Ccnd2, Ccnd3, Ccnb2, Cdc42, Cdk6, Cdk4, Csnk2a1, Csnk2b |
| Others | Egfr, Ksr1, Col1a1, Hspa5a, Hspb1 |
| WNT signaling | |
| WNT ligands and receptors | Wnt 2b, Wnt3, Wnt3a, Wnt4, Wnt7b, Frzb, Fzd2, Fzd5a, Fzd6 |
| APC complex | Dvl1, Axin1, Axin2, Ctnnb1 |
| Non-canonical WNT signaling | Wnt5a, Fzd6, Dvl1, RhoA, Nkd1, Nkd2 |
| Others | Tcf3, Tcf4, Tle1, RhoA, Bcl9, Dkk3, Nkd2, Sfrp1, Sfrp4, Fbxw2, Lef1, Lrp5, Pitx2, Porcn, Rgd1561440, Ppp2ca |
| TGF-β Signaling | |
| Growth factors | Smad1, Smad2, Smad5 |
| Transcription regulators | Tgfb1, Tgfb2, Tgfb3, Bmp1, Bmp4, Bmpr2, Inhba |
| Kinases | Tgfbr1, Tgfbr2, Tgfbr3 |
| Other genes important in hCRC | |
| Transcription regulators | Klf4a, Sox4, Sox9, Stat1, Stat3, Tcf7l2 |
| Kinases | Pik3cb, Pik3r1, Pik3r2, Akt1, Akt2, Akt3, Fgfr1, Sgk1a, Stk11, Fgfr1 |
| Phosphatases | Cdc25a, Dusp4, Ptpro, Ptprs, |
| Apoptosis | Birc5, Bax, Casp3a |
| Others | Tnf-α, Nos2, Ca2a, Prdx6, Hpgda, Hsd17b2a, Msh2, Psat1, Timp1a, Hspd1, Top2a, Sparc |
a
Down-regulated gene. All other genes are up-regulated compared to untreated control colonic epithelium.