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. Author manuscript; available in PMC: 2017 Jun 15.
Published in final edited form as: Neuron. 2016 Jun 2;90(6):1174–1188. doi: 10.1016/j.neuron.2016.05.005

Figure 6. Expression of LncND in neural progenitor cells including radial glia cells in VZ and OSVZ in human neocortex.

Figure 6

(A) Single-cell RNA-seq analysis from human cerebral organoids–Heatmap (Pearson’s correlation), representing the clustering of cells based on the known radial glia cell markers. LncND-expressing cells are represented in purple on top of the heatmap. Genes marked in red box represents example of DE genes between LncND-cluster (marked in black box) and the rest of the cells. (B) Left and middle panel: GW17 primary human neocortex section demonstrating the PAX6+ radial glia cells and nuclear staining with DAPI. Right panel: in-situ hybridization with a probe specific for LncND showing its localization in VZ and OSVZ progenitor cells. Experiment was repeated at least two times. (C) Co-localization of LncND and PAX6+ cells in the OSVZ representing outer radial glia cells (oRG). Red and green boxes of the merged figure are zoomed-in to show the co-localization of LncND and PAX6. Cells were immunostained with PAX6-specific antibody (red) after performing in-situ hybridization with LncND-specific probe (green dots). Experiment was repeated at least two times. Scale: 50μm.