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. Author manuscript; available in PMC: 2017 Jun 15.
Published in final edited form as: Neuron. 2016 Jun 2;90(6):1272–1285. doi: 10.1016/j.neuron.2016.05.004

Fig. 5. Palmitoleic acid is a stimulatory pheromone that promotes male courtship.

Fig. 5

Single-pair courtship assays with a 7-day old naïve male and a 2-day old virgin female. Cumulative copulation rates are plotted against time (left panels). Final copulation rates are shown with lines connecting results from parallel experiments (right panels). (A) Copulation rates of Or47b mutant males were significantly lower than that of WT controls. (B) Genetic rescue of Or47b mutation restored the copulation rate. Data were from 6 experiments, 13 to 30 pairs of flies per experiment, 149–164 pairs per genotype. Significant differences (p<0.05) are denoted by different letters, ANOVA followed by Tukey’s test. (C–D) Perfuming the mating chamber with palmitoleic acid (PA) increased the copulation rate of WT (C) but not Or47b2 mutant males (D). Solvent control: ethanol. Results are from 5 parallel experiments, 19–25 pairs of flies per experiment, 119–125 pairs per condition. Paired t-test, **, p<0.01.