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. Author manuscript; available in PMC: 2017 Jun 15.
Published in final edited form as: Cancer Res. 2016 Apr 12;76(12):3473–3483. doi: 10.1158/0008-5472.CAN-15-1742

Figure 1. Generation of iPSCs from Pmel-1 TCR transgenic CD8+ T cells.

Figure 1

(A) Schematic illustration showing the generation of iPSCs from Pmel-1 TCR transgenic CD8+ T cells. (B) Efficient GFP introduction by Sendai virus vector in Pmel-1 CD8+ T cells transfected at an MOI of 20. (C) Morphology, alkaline phosphatase (ALP) activity and expression of pluripotency and surface markers (SSEA1, Oct3/4 and Nanog) in Pmel-1 iPSCs 1 and 2, and mESCs. Nuclei were counterstained with DAPI or HOECHST. Scale bar: 200 μm. (D) RT-PCR analysis for the ESC marker genes, Nanog, Oct3/4, Sox2, and Ecat1 in Pmel-1 iPSCs and mESCs. (E) RT-PCR analysis for the transgenes, Oct3/4 Sox2, Klf4, c-Myc and Sendai virus in Pmel-1 iPSCs.