Table 1.
The sequence of rearranged TCRβ chain genes and frequency in Pmel-1 iPSC-derived DP, CD8 SP on OP9-DL1 cells and splenic CD8+ T cells.
| Cell type | Productive rearrangement | Productive frequency | Frequency | Amino acid |
|---|---|---|---|---|
| Pmel-1 iPSC-CD8+ T cells | TCRBV14-01*01 TCRBD01-01*01 TCRBJ01-06*01 |
97.8% | 93.2% | CASSFHRDYNSPLYF |
| Pmel-1 splenic CD8+ T cells | TCRBV14-01*01 TCRBD01-01*01 TCRBJ01-06*01 |
97.8% | 95.0% | CASSFHRDYNSPLYF |
| Cell type | Nucleotide |
|---|---|
| Pmel-1 iPSC- CD8+ T cells | CTCAAGATCCAGTCTGCAAAGCAGGGCGACACAGCCACCTATC TCTGTGCCAGCAGTTTCCACAGGGACTATAATTCGCCCCTCTAC |
| Pmel-1 splenic CD8+ T cells |
CTCAAGATCCAGTCTGCAAAGCAGGGCGACACAGCCACCTATC TCTGTGCCAGCAGTTTCCACAGGGACTATAATTCGCCCCTCTAC |