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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Appl Microbiol Biotechnol. 2016 Mar 12;100(13):5883–5895. doi: 10.1007/s00253-016-7431-x

Fig.9.

Fig.9

Electron microscopy of the three recombinant yeast strains. The cells were rapidly frozen after methanol induction for 96 h. Following freeze-substitution, infiltration and polymerization, the sample blocks were serially sectioned to a thickness of about 100 nm. The structural preservation is very good as demonstrated in the whole-cell micrographs in a GS115/CALB-GCW51, b GS115/SC3-61/CALB-51, and c GS115-51/HFBI-61/CALB-51. The cell wall regions, marked by the dash-line squares in the left figure column, are further magnified in d GS115/CALB-GCW51, e GS115/SC3-61/CALB-51 and f GS115-51/HFBI-61/CALB-51, respectively. The difference of the inner layers and the outer mannan fibrils are visible among the three strains.