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. Author manuscript; available in PMC: 2017 Jun 15.
Published in final edited form as: Cancer Res. 2016 Apr 13;76(12):3572–3582. doi: 10.1158/0008-5472.CAN-15-2959

Figure 2. Ad.5/3-CTV induces apoptosis in neuroblastoma cells.

Figure 2

(A) Neuroblastoma cells were cultured in 8-well chamber slide and treated with 25 pfu of Ad.5/3-Null or Ad.5/3-E1A or the indicated dose of Ad.5/3-CTV for 72 hours. Cells were fixed and TUNEL assays were performed. Data presented as TUNEL positive cells in a defined microscopic field as compared with un-treated control cells. (B) Neuroblastoma cells were infected as described in 2A for 72 hours and were collected and subjected to FACS analysis with propidium iodide staining for DNA content and data presented in a graphical manner from three independent experiments. Columns: mean of triplicate experiments. Bars: S.D., *, p<0.001 versus control. (C) Neuroblastoma cells were treated as described in 2A for 72 hours. Cells were collected and western blot analysis was performed for PARP using specific antibody and β-Actin served as loading control.