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. Author manuscript; available in PMC: 2017 Jun 15.
Published in final edited form as: Cancer Res. 2016 Apr 13;76(12):3572–3582. doi: 10.1158/0008-5472.CAN-15-2959

Figure 3. Ad.5/3-CTV induces caspase-independent cell death in neuroblastoma cells.

Figure 3

(A) Neuroblastoma cells were infected with 25 pfu of Ad.5/3-Null or Ad.5/3-E1A or with the indicated dose of Ad.5/3-CTV for 72 hours. Cells were collected and western blot analysis was performed for caspase-3 and caspase-9 using specific antibodies and β-Actin served as loading control. Staurosporine served as a positive control for caspase 3 activation. (B) Neuroblastoma cells were treated as described in 3A for 72 hours, collected and caspase-3 activation assays were performed according to the manufacturer’s protocol. Staurosporine served as a positive control. Results represent three independent experiments displayed in a graphical manner. Columns: mean of triplicate experiments; bars, S.D. (C) Neuroblastoma cells were pre-treated with 20 μM Z-VAD-FMK and were infected as described in 3A for 72 hours. Cells were collected and western blotting analysis was performed for PARP using specific antibody and β-Actin served as loading control. Results are representative of three independent experiments.