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. Author manuscript; available in PMC: 2016 Dec 15.
Published in final edited form as: Cancer Res. 2016 Apr 13;76(12):3520–3530. doi: 10.1158/0008-5472.CAN-15-3465

Figure 3. MYC reduction potentiates TGFβ-induced upregulation of integrin αvβ3 and an invasive cellular phenotype.

Figure 3

(A–B) TGFβ treatment and MYC knockdown of MCF10A cells stimulate integrin αvβ3 through a potentiation effect that is dependent upon expression of integrin αvβ3, assessed by protein expression (A) and cell invasion (B). (C–E) MCF10A cells continuously exposed to TGFβ or exposed for a short period of time and then cultured in the absence of exogenous TGFβ showed persistent expression of MYC, integrin αv and integrin β3 (C) and TGFβ-responsive reporter construct (D) through consistent activation of TGFβ expression (E). Transcriptional results are presented as relative quantification, normalized to GAPDH. Error bars, SEM; * p<0.5; ** p<0.01; *** p<0.001.