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. Author manuscript; available in PMC: 2016 Nov 18.
Published in final edited form as: Nature. 2016 May 18;534(7607):347–351. doi: 10.1038/nature17964

Extended Data Figure 1. Reconstitution of TRPV1 into lipid nanodisc.

Extended Data Figure 1

a, Size exclusion chromatography of TRPV1 channel reconstituted into lipid nanodisc using MSP2N2. Void volume and peaks corresponding to TRPV1 and cleaved MBP are indicated. b, SDS-PAGE of detergent solubilized MBP-TRPV1 fusion protein and material from nanodisc reconstituted with TRPV1 following MBP cleavage (middle peak in a). Note the presence of both bands for TRPV1 and MSP2N2. c, Representative micrograph of negative-stained TRPV1-nanodisc sample showing mono-dispersed and homogeneous particles. d, Reference-free 2D class averages of particles in (c) revealing band-like density contributed by the lipid disc (side view) and tetrameric arrangement of channel subunits (top view). e, 2D class averages of the same protein reconstituted into MSP1E3 nanodisc, which is smaller in diameter. Note the extra space within the disc offered by MSP2N2 scaffold protein in (d).