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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Inflamm Bowel Dis. 2016 Jul;22(7):1587–1595. doi: 10.1097/MIB.0000000000000840

Figure 6.

Figure 6

Macrophage migration assay. Images of marcophages on trans-well membranes after exposure of macrophages to EVs from IBD patients labelled with acridine orange (red), and the nucleic acids of macrophages were stained with Hoechst 33258 (blue) (A, 20× magnification); (B) Mean cell counts on the trans-well membrane after 10 hours of exposure of macrophages to serum-free medium (negative control), 10% FBS (positive control), EVs from IBD patients (IBD EV), DLD-1 cells alone, or DLD-1 cells cultured with IBD EVs. Note that error bars are standard deviations.