Figure 1.

CRFR1 in the Arc Is Expressed in Neurons Regulating Food Intake and Growth

(A) Double in situ hybridization for Crfr1 and Gfp on CRFR1-GFP mice.

(B) Immunodetection of GFP in the Arc of CRFR1-GFP mice crossed with AgRP reporter mice (CRFR1-GFP-AgRP^TOM).

(C) Immunodetection of GFP and AgRP in the Arc of colchicine injected CRFR1-GFP mice crossed with POMC reporter mice (CRFR1-GFP-POMC^TOM).

(D) Immunodetection of GFP and ACTH in the Arc of CRFR1-GFP mice.

(E) Immunodetection of GFP in the Arc of CRFR1-GFP mice crossed with RIP reporter mice (CRFR1-GFP-RIP^TOM).

(F) Immunodetection of GFP and SST in the Arc of colchicine injected CRFR1-GFP mice.

(G) Immunodetection of GFP and TH in the Arc of colchicine injected CRFR1-GFP mice.

(H) Percentage of each CRFR1 subpopulation from total Arc-CRFR1-expressing neurons. Values are mean ± SEM. ND, not detected. AgRP n = 7 for each sex; POMC-Cre n = 3; ACTH n = 2; RIP n = 2; SST n = 2; TH n = 2.

(I) Percentage of CRFR1 subpopulation from total AgRP-expressing neurons. Values are mean ± SEM (n = 7 for each sex).