Figure 2.

CRFR1 Marks AgRP Neurons Originating from POMC Precursor

(A) Immunodetection of GFP and ACTH in the Arc of colchicine injected CRFR1-GFP mice crossed with POMC reporter mice (CRFR1-GFP-POMC^TOM).

(B) Immunodetection of GFP and AgRP in the Arc of colchicine injected CRFR1-GFP mice crossed with POMC reporter mice (CRFR1-GFP-POMC^TOM).

(C) Pomc and Agrp relative expression in 4-day-old CRFR1 KO mice and their WT littermates (n = 4 or 5).

(D) Immunodetection of pSTAT3 in the Arc of leptin- or saline-injected, overnight-fasted CRFR1-GFP-AgRP^TOM reporter mice.

(E) Immunodetection of pSTAT3 in the Arc of leptin-injected, overnight-fasted CRFR1-GFP-POMC^TOM reporter mice.

(F) Venn diagrams displaying the percentage of unique and overlapping leptin responsive CRFR1 and AgRP neurons in males and females (n = 3 for each sex). See also Figure S1E.