CRFR1 Marks AgRP Neurons Originating from POMC Precursor
(A) Immunodetection of GFP and ACTH in the Arc of colchicine injected CRFR1-GFP mice crossed with POMC reporter mice (CRFR1-GFP-POMCTOM).
(B) Immunodetection of GFP and AgRP in the Arc of colchicine injected CRFR1-GFP mice crossed with POMC reporter mice (CRFR1-GFP-POMCTOM).
(C) Pomc and Agrp relative expression in 4-day-old CRFR1 KO mice and their WT littermates (n = 4 or 5).
(D) Immunodetection of pSTAT3 in the Arc of leptin- or saline-injected, overnight-fasted CRFR1-GFP-AgRPTOM reporter mice.
(E) Immunodetection of pSTAT3 in the Arc of leptin-injected, overnight-fasted CRFR1-GFP-POMCTOM reporter mice.
(F) Venn diagrams displaying the percentage of unique and overlapping leptin responsive CRFR1 and AgRP neurons in males and females (n = 3 for each sex). See also Figure S1E.